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Dynamic Expression and Heterogeneous Intracellular Location of En-1 during Late Mouse Embryonic Development

Authors
  • Zhong, Shan-chuan
  • Chen, Xing-shu
  • Cai, Qi-yan
  • Luo, Xue
  • Chen, Xing-hua
  • Liu, Jing
  • Yao, Zhong-xiang
Type
Published Article
Journal
Cells Tissues Organs
Publisher
S. Karger AG
Publication Date
Nov 19, 2009
Volume
191
Issue
4
Pages
289–300
Identifiers
DOI: 10.1159/000260063
PMID: 19940436
Source
Karger
Keywords
License
Green
External links

Abstract

Engrailed-1 (En-1) is a transcription factor involved in the development of the midbrain/hindbrain during mouse early embryogenesis. Although En-1 is expressed from embryogenesis to adulthood, there has been no detailed description of its expression during late mouse embryonic development. Here we report the expression pattern of En-1 in the mouse embryo from E10.5 to the neonatal state. With immunohistochemistry we found that En-1 was expressed in the central nervous system (CNS) from E10.5 to the neonatal state, mostly restricted to the midbrain/hindbrain junction. Outside the CNS, En-1 is dynamically expressed in several neural crest-associated structures including the cranial mesenchyme, the mandibular arches, the vagus nerve, the dorsal root ganglia, the sympathetic ganglia, the somites, the heart and the cloaca. Additionally, we found that in the CNS, most of the En-1 was located in the nuclei, while outside the CNS, En-1 was mainly expressed in the cytoplasm. These findings provided additional evidence that En-1 may be involved in the development of neural crest cells.

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