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Duplexed sandwich immunoassays on a fiber-optic microarray

Authors
  • Rissin, David M.
  • Walt, David R.
Type
Published Article
Journal
Analytica Chimica Acta
Publisher
Elsevier
Publication Date
Jan 01, 2005
Accepted Date
Oct 08, 2005
Volume
564
Issue
1
Pages
34–39
Identifiers
DOI: 10.1016/j.aca.2005.10.012
Source
Elsevier
Keywords
License
Unknown

Abstract

In this paper, we describe a duplexed imaging optical fiber array-based immunoassay for immunoglobulin A (IgA) and lactoferrin. To fabricate the individually addressable array, microspheres were functionalized with highly specific monoclonal antibodies. The microspheres were loaded in microwells etched into the distal face of an imaging optical fiber bundle. Two microsphere-based sandwich immunoassays were developed to simultaneously detect IgA and lactoferrin, two innate immune system proteins found in human saliva. Individual microspheres could be interrogated for the simultaneous measurement of both proteins. The working concentration range for IgA detection was between 700 pM and 100 nM, while the working concentration range for lactoferrin was between 385 pM and 10 nM. The cross-reactivity between detection antibodies and their non-specific targets was relatively low in comparison to the signal generated by the specific binding with their targets. These results suggest that the degree of multiplexing on this fiber-optic array platform can be increased beyond a duplex.

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