Affordable Access

Publisher Website

Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro.

  • Saukko, Annina E A1, 2
  • Nykänen, Olli1, 3
  • Sarin, Jaakko K1, 4
  • Nissi, Mikko J1, 3
  • Te Moller, Nikae C R5
  • Weinans, Harrie6, 7
  • Mancini, Irina A D5
  • Visser, Jetze7
  • Brommer, Harold5
  • van Weeren, P Réné5
  • Malda, Jos5, 7
  • Grinstaff, Mark W8
  • Töyräs, Juha1, 4, 9, 10
  • 1 Department of Applied Physics, University of Eastern Finland, Kuopio, Finland. , (Finland)
  • 2 Department of Medical Physics, Turku University Hospital, Turku, Finland. , (Finland)
  • 3 Research Unit of Medical Imaging Physics and Technology, University of Oulu, Oulu, Finland. , (Finland)
  • 4 Diagnostic Imaging Center, Kuopio University Hospital, Kuopio, Finland. , (Finland)
  • 5 Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. , (Netherlands)
  • 6 Department of Biomechanical Engineering, Delft University of Technology (TU Delft), Delft, The Netherlands. , (Netherlands)
  • 7 Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands. , (Netherlands)
  • 8 Departments of Biomedical Engineering, Chemistry, and Medicine, Boston University, Boston, Massachusetts, USA.
  • 9 School of Information Technology and Electrical Engineering, The University of Queensland, Brisbane, Australia. , (Australia)
  • 10 Science Service Center, Kuopio University Hospital, Kuopio, Finland. , (Finland)
Published Article
Journal of Orthopaedic Research®
Wiley (John Wiley & Sons)
Publication Date
May 12, 2021
DOI: 10.1002/jor.25066
PMID: 33982283


To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning. Posttraumatic changes include proteoglycan (PG) loss and elevated water content. Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes. Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture. Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies. Control samples were collected from three nonoperated ponies. The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points. As a reference, equilibrium moduli, dynamic moduli, and PG content were measured. Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min. Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content. QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries. © 2021 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.

Report this publication


Seen <100 times