Affordable Access

deepdyve-link
Publisher Website

Dual near-infrared fluorescence-based lateral flow immunosensor for the detection of zearalenone and deoxynivalenol in maize.

Authors
  • Jin, Yongpeng1
  • Chen, Qian2
  • Luo, Sunlin1
  • He, Lidong3
  • Fan, Ruiqi1
  • Zhang, Siwei1
  • Yang, Chunjiang4
  • Chen, Yiqiang1
  • 1 State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China. , (China)
  • 2 City of Hope National Medical Center, Duarte, CA, USA.
  • 3 Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, USA.
  • 4 Ring Biotechnology Co. Ltd., Beijing, China. , (China)
Type
Published Article
Journal
Food chemistry
Publication Date
Aug 01, 2020
Volume
336
Pages
127718–127718
Identifiers
DOI: 10.1016/j.foodchem.2020.127718
PMID: 32763741
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

A novel dual near-infrared fluorescence-based lateral flow immunosensor was developed to determine zearalenone and deoxynivalenol in maize. Two near-infrared dyes with distinct fluorescence characteristics were utilized to separately label the anti-zearalenone and anti-deoxynivalenol antibodies as detection reagents. The capture antigens zearalenone-BSA and deoxynivalenol-BSA were mixed and immobilized on the same test line of nitrocellulose membrane. This assay format facilitates simultaneous detection of the two mycotoxins on a single test line. After optimizing experimental parameters, the limits of detection for zearalenone and deoxynivalenol were as low as 0.55 μg/kg and 3.8 μg/kg in maize, respectively. The spiking experiment yielded recovery ratios ranging from 81.7% to 107.3% with coefficients of variation less than 14% demonstrating high assay accuracy and precision. Moreover, the actual sample analysis produced consistent results between this method and instrumental method. Therefore, the developed immunosensor can serve as an accurate and efficient approach for monitoring mycotoxins in agricultural products. Copyright © 2020 Elsevier Ltd. All rights reserved.

Report this publication

Statistics

Seen <100 times