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Dual mechanical impact of β-escin on model lipid membranes

Authors
  • Moleiro, Lara H.1, 2
  • Martín-Romero, María T.3
  • Herráez-Aguilar, Diego4
  • Santiago, José A.5
  • Caselli, Niccolò1, 2
  • Dargel, Carina2, 6
  • Geisler, Ramsia2, 7
  • Hellweg, Thomas2
  • Monroy, Francisco1, 8
  • 1 Universidad Complutense de Madrid, Madrid , (Spain)
  • 2 Universität Bielefeld, Bielefeld , (Germany)
  • 3 Universidad de Córdoba, Córdoba , (Spain)
  • 4 Universidad Francisco de Vitoria (UFV), Madrid , (Spain)
  • 5 Universidad Autónoma Metropolitana Cuajimalpa, Ciudad de México , (Mexico)
  • 6 Universität Münster, Münster , (Germany)
  • 7 Goethe-Universität, Frankfurt , (Germany)
  • 8 Hospital 12 de Octubre (imas12), Madrid , (Spain)
Type
Published Article
Journal
Frontiers in Soft Matter
Publisher
Frontiers Media S.A.
Publication Date
Sep 08, 2023
Volume
3
Identifiers
DOI: 10.3389/frsfm.2023.1240878
Source
Frontiers
Keywords
Disciplines
  • Soft Matter
  • Original Research
License
Green

Abstract

Understanding the mechanical behavior of biological membranes is of paramount importance in cell biophysics and in developing new biomaterials for medicine. In this study, we delve into the mechanical impact of β-escin, commonly referred to as escin, a naturally occurring biosurfactant derived from the seeds of the horse chestnut tree. To examine the modulable interaction between escin and dimyristoylphosphatidylcholine (DMPC), which is an archetypical fluid phospholipid and an essential constituent of the cellular fluid membrane, we have used artificial models based on the liquid crystal structure, such as bilayer vesicles and Langmuir monolayers. We have focused on the energetic and kinetic aspects of escin insertion when transversally adsorbed or longitudinally integrated within these model membranes. By employing surface microscopies of epifluorescence and Brewster angle reflectivity, we have elucidated the structural phase behavior of hybrid escin–phospholipid membranes, which exhibit dual mechanical properties characterized by high rigidity and reduced fluidity. Notably, at low temperatures, we observe a soft, glassy rheological behavior reminiscent of liquid crystalline ordered phases, which turns into a fluid-like viscoelasticity resembling more disordered phases at physiological temperatures. The hybrid membranes behave in one way or another as both are driven by an adsorption potential well imposed by escin cohesivity. These intriguing findings are discussed from a physicochemical perspective, highlighting their potential for future pharmacological designs and biomedical applications that exploit the dual mechanical impact of escin on biological membranes.

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