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Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP.

Authors
  • Salvaterra, P M
  • Kitamoto, T
Type
Published Article
Journal
Brain research. Gene expression patterns
Publication Date
Aug 01, 2001
Volume
1
Issue
1
Pages
73–82
Identifiers
PMID: 15018821
Source
Medline
License
Unknown

Abstract

Using 7.4 kb of 5' flanking DNA from the Drosophila cholinergic gene locus to drive Gal4 expression we can visualize essentially all cholinergic neurons and neuropiles after genetic recombination with a UAS-GFP (S65T) reporter gene. In contrast to previous methods somata and neuropiles can be observed in the same samples. Fluorescence intensity is strong enough to allow observations in live animals at all developmental stages. Three-dimensional reconstructions made from confocal sections of whole-mount preparations reveal the extensive cholinergic connections among various regions of the nervous system.

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