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Drivers of molecular and morphometric variation in Triatoma brasiliensis (Hemiptera: Triatominae): the resolution of geometric morphometrics for populational structuring on a microgeographical scale

Authors
  • Kamimura, Edgard H.1
  • Viana, Maria Carolina1
  • Lilioso, Maurício1
  • Fontes, Fernanda H. M.1
  • Pires-Silva, Dayane1
  • Valença-Barbosa, Carolina1
  • Carbajal-de-la-Fuente, Ana L.2, 3
  • Folly-Ramos, Elaine4
  • Solferin, Vera N.1
  • Thyssen, Patricia J.1
  • Costa, Jane5
  • Almeida, Carlos E.1
  • 1 Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil , Campinas (Brazil)
  • 2 Centro Nacional de Diagnóstico e Investigación en Endemo-Epidemias (CeNDIE), Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán” (ANLIS), Buenos Aires, Argentina , Buenos Aires (Argentina)
  • 3 Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina , Buenos Aires (Argentina)
  • 4 Universidade Federal da Paraíba (UFPB), Campus IV, Rio Tinto, Brazil , Rio Tinto (Brazil)
  • 5 Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/Fiocruz-RJ), Rio de Janeiro, Brazil , Rio de Janeiro (Brazil)
Type
Published Article
Journal
Parasites & Vectors
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Sep 07, 2020
Volume
13
Issue
1
Identifiers
DOI: 10.1186/s13071-020-04340-7
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundThe protozoan Trypanosoma cruzi circulates in semiarid areas of northeastern Brazil in distinct ecotopes (sylvatic, peridomestic and domestic) where Triatoma brasiliensis Neiva, 1911 is the most important Chagas disease vector. In this study, we analyzed microevolutionary and demographic aspects of T. brasiliensis populations at the ecotypic, micro and macro-geographic scales by combining morphometrics and molecular results. Additionally, we aimed to address the resolution of both markers for delimiting populations in distinct scales.MethodsWe sampled populations of T. brasiliensis from distinct ecotypic and geographic sites in the states Rio Grande do Norte (RN) and Paraíba (PB). The geometric morphometry was carried out with 13 landmarks on the right wings (n = 698) and the genetic structure was assessed by sequencing a region of cytochrome b mitochondrial gene (n = 221). Mahalanobis distance (MD) and coefficient of molecular differentiation (ΦST) were calculated among all pairs of populations. The results of comparisons generated MD and ΦST dendrograms, and graphics of canonical variate analysis (CVA).ResultsLittle structure was observed for both markers for macro-geographic scales. Mantel tests comparing geographic, morphometric and genetic matrices showed low correlation (all R2 < 0.35). The factorial graphics built with the CVA evidenced population delimitation for the morphometric data at micro-geographic scales.ConclusionsWe believe that T. brasiliensis carries in its genotype a source of information to allow the phenotypical plasticity across its whole distribution for shaping populations, which may have caused a lack of population delimitation for CVAs in morphometric analysis for macro-geographic scale analysis. On the other hand, the pattern of morphometric results in micro-geographic scales showed well-defined groups, highlighting the potential of this tool to inferences on the source for infestation.

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