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Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide.

Authors
  • Triskova, Iveta1
  • Veznik, Jakub2
  • Lacina, Karel3
  • Trnkova, Libuse4
  • 1 Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic. , (Czechia)
  • 2 Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic; CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic. , (Czechia)
  • 3 CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic. , (Czechia)
  • 4 Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic. Electronic address: [email protected] , (Czechia)
Type
Published Article
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
Publication Date
Mar 14, 2020
Volume
134
Pages
107515–107515
Identifiers
DOI: 10.1016/j.bioelechem.2020.107515
PMID: 32200262
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In this paper, the d(GCGAAGC) heptamer and the closely related d(GCGAGC) hexamer are examined via electrochemical (cyclic voltammetry) and spectroscopic (circular dichroism) methods. Dramatic changes in the CD spectroscopic and CV electrochemical properties, induced by the loss of only one single nucleotide (A), are detected. The CD spectra and native polyacrylamide gel electrophoresis (PAGE) confirmed structural changes taking place in the relevant chain-like oligodeoxynucleotide assemblies. Dedicated studies suggest that the heptamer (Hp) possesses a hairpin structure, whereas the hexamer (Hx) appears to be rather a duplex. Both of the structures exhibited completely different adsorption behavior at the hanging mercury drop electrode, and this factor was readily confirmed by means of elimination voltammetry with linear scan (EVLS). We established that the Hp hairpin (~-1300 mV), compared to the Hx duplex (~-1360 mV), is the thermodynamically favored electron acceptor. The adsorption isotherms were constructed based on the voltammetric peak height values, reflecting the reduction of the adenine (A) and cytosine (C) moieties as well as the oxidation of the 7,8-dihydroguanine (7,8-DHG) moieties. Finally, as revealed by the spectroscopic and electrochemical results, Hx forms a bimolecular antiparallel homo-duplex carrying both Watson-Crick base pairs (CG or GC) and mismatched edge-to-edge base pairs (GA or AG). Copyright © 2020. Published by Elsevier B.V.

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