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The draft genome sequence of “Nitrospira lenta” strain BS10, a nitrite oxidizing bacterium isolated from activated sludge

Authors
  • Sakoula, Dimitra1
  • Nowka, Boris2
  • Spieck, Eva2
  • Daims, Holger3
  • Lücker, Sebastian1
  • 1 Radboud University, Department of Microbiology, IWWR, Heyendaalseweg 135, Nijmegen, 6525 AJ, Netherlands , Nijmegen (Netherlands)
  • 2 University of Hamburg, Department of Microbiology & Biotechnology, Ohnhorststr. 18, Hamburg, 22609, Germany , Hamburg (Germany)
  • 3 University of Vienna, Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, Althanstr. 14, Vienna, 1090, Austria , Vienna (Austria)
Type
Published Article
Journal
Standards in Genomic Sciences
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Nov 22, 2018
Volume
13
Issue
1
Identifiers
DOI: 10.1186/s40793-018-0338-7
Source
Springer Nature
Keywords
License
Green

Abstract

The genus Nitrospira is considered to be the most widespread and abundant group of nitrite-oxidizing bacteria in many natural and man-made ecosystems. However, the ecophysiological versatility within this phylogenetic group remains highly understudied, mainly due to the lack of pure cultures and genomic data. To further expand our understanding of this biotechnologically important genus, we analyzed the high quality draft genome of “Nitrospira lenta” strain BS10, a sublineage II Nitrospira that was isolated from a municipal wastewater treatment plant in Hamburg, Germany. The genome of “N. lenta” has a size of 3,756,190 bp and contains 3968 genomic objects, of which 3907 are predicted protein-coding sequences. Thorough genome annotation allowed the reconstruction of the “N. lenta” core metabolism for energy conservation and carbon fixation. Comparative analyses indicated that most metabolic features are shared with N. moscoviensis and “N. defluvii”, despite their ecological niche differentiation and phylogenetic distance. In conclusion, the genome of “N. lenta” provides important insights into the genomic diversity of the genus Nitrospira and provides a foundation for future comparative genomic studies that will generate a better understanding of the nitrification process.

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