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Downregulation of pro-inflammatory cytokines by lupeol measured using cytometric bead array immunoassay.

Authors
  • Ahmad, Sheikh Fayaz
  • Pandey, Anjali
  • Kour, Kiranjeet
  • Bani, Sarang
Type
Published Article
Journal
Phytotherapy Research
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jan 01, 2010
Volume
24
Issue
1
Pages
9–13
Identifiers
DOI: 10.1002/ptr.2844
PMID: 19548206
Source
Medline
License
Unknown

Abstract

The objective of the study was to investigate the activity of Lupeol (LUP) on proinflammatory and anti-inflammatory cytokines in the pleural exudate from male swiss albino mice. We applied Cytometric bead array technology for simultaneously measurement of these cytokines in pleurisy induced mice treated with lupeol in graded oral doses. Cytometric bead array uses the sensitivity of amplified fluorescence detection by flowcytometer to measure soluble analytes in a particle based immune assay. This assay can accurately quantitate 5 cytokines in a 50 microlitre sample volume. Oral administration of LUP at doses of 25, 50, 100 and 200 mg/kg p.o. produced dose related inhibition of IL-2, IFN-gamma and TNF-alpha in the pleural exudate with the most significant effect at 100 mg/kg oral dose. LUP had a non significant inhibitory effect on the levels of IL-4 and IL-5.

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