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Down regulation of the mercury resistance operon by the most promoter-distal gene merD.

Authors
Type
Published Article
Journal
Molecular & general genetics : MGG
Publication Date
Volume
220
Issue
1
Pages
69–72
Identifiers
PMID: 2691875
Source
Medline
License
Unknown

Abstract

The effect of the merD gene on the expression of the mer operon was determined from the rates of accumulation of merA-lacZ fusion protein in the presence and absence of an active merD gene in trans. In the presence of the merD gene, beta-galactosidase activity was 2- to 4-fold lower. The merD gene was cloned in a T7 promoter expression vector and the MerD protein product was visualized by autoradiography.

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