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Double Jeopardy: Both Overexpression and Suppression of a Redox-Activated Plant Mitogen-Activated Protein Kinase Render Tobacco Plants Ozone Sensitive

Authors
  • Marcus A. Samuel
  • Brian E. Ellis
Publisher
American Society of Plant Biologists
Publication Date
Sep 01, 2002
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Double Jeopardy by Marcus A. Samuel, and Brian E. Ellis Plantcell Volume 14(9):2059-2069 September 1, 2002 ©2002 by American Society of Plant Biologists Post-Transcriptional Gene Silencing-Induced Suppression of Cell Death Triggered by Transient Overexpression of SIPK.Coinfiltration with Agrobacterium containing the SIPK-FLAG construct along with the SIPK-RI construct inhibited the cell death process induce... Samuel M, Ellis B. Plantcell 2002;14:2059-2069 ©2002 by American Society of Plant Biologists Transgenic Tobacco Plants Overexpressing SIPK-FLAG Show Increased Ozone Sensitivity.(A) Proteins (40 μg) extracted from leaves of the different OX lines ectopically expressing SIPK-FLAG were immunoblotted using anti-FLAG antibody.(B) Transgenic tobacco line... Samuel M, Ellis B. Plantcell 2002;14:2059-2069 ©2002 by American Society of Plant Biologists SIPK-Suppressed Lines Also Are Sensitive to Ozone.(A) RNAi construct under the control of the 35S promoter of Cauliflower mosaic virus.(B) SIPK is suppressed in four of the six PCR-positive lines. Samuel M, Ellis B. Plantcell 2002;14:2059-2069 ©2002 by American Society of Plant Biologists Quantitation of Ozone-Induced Cell Death and Hydrogen Peroxide Accumulation in SIPK Kinotypes.(A) Ion leakage from leaf discs (five each) of the third and fourth leaves of wild-type, OX4, and RI5 lines was assessed as an indicator of the loss of membrane in... Samuel M, Ellis B. Plantcell 2002;14:2059-2069 ©2002 by American Society of Plant Biologists Activity and Expression of Mutagenized SIPK.(A) The recombinant SIPK activation loop mutant is less active than wild-type SIPK. Myelin basic protein (MBP)-phosphorylating activities of SIPK and SIPK(AEF) were measured by incubating recombinant proteins (5 μ... Samuel M, Ellis B. Plantcell 2002;14:2059-2069 ©2002 by American Society of Plant Biologists Differential Ozone-Induced Activation of SIPK and WIPK in SIPK Kinotypes.(A) Crude protein extracts prepared from ozone-exposed tissues from T1 lines of the d

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