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Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene.

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PMC
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  • Research Article

Abstract

The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.

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