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Does conserved domain SOD1 mutation has any role in ALS severity and therapeutic outcome?

Authors
  • Pal, Surinder1
  • Tiwari, Abha2
  • Sharma, Kaushal3
  • Sharma, Suresh Kumar4
  • 1 Centre for Systems Biology and Bioinformatics, Panjab University, Chandigarh, India , Chandigarh (India)
  • 2 Goa University, Taleigao Plateau, Goa, India , Taleigao Plateau, Goa (India)
  • 3 Advanced Pediatrics Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India , Chandigarh (India)
  • 4 Department of Statistics, Panjab University, Chandigarh, India , Chandigarh (India)
Type
Published Article
Journal
BMC Neuroscience
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Oct 09, 2020
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s12868-020-00591-3
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundAmyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative fatal disease that can affect the neurons of brain and spinal cord. ALS genetics has identified various genes to be associated with disease pathology. Oxidative stress induced bunina and lewy bodies formation can be regulated through the action of SOD1 protein. Hence, in the present study we aim to analyse the structural and functional annotation of various reported SOD1 variants throughout and their putative correlation with the location of mutation and degree of ALS severity by inferring the structural and functional alterations in different SOD1 variants.MethodsWe have retrieved around 69 SNPs of SOD1 gene from Genecards. Structural annotation of SOD1 variants were performed using SWISS Model, I-Mutant 2.0, Dynamut, ConSurf. Similarly, the functional annotation of same variants were done using SIFT, PHP-SNP, PolyPhen2, PROVEAN and RegulomeDB. Ramachandran plot was also obtained for six synonymous SNPs to compare the amino acid distribution of wild-type SOD1 (WT SOD1) protein. Frequency analysis, Chi square analysis, ANOVA and multiple regression analysis were performed to compare the structural and functional components among various groups.Results and conclusionResults showed the mutations in conserved domain of SOD1 protein are more deleterious and significantly distort the tertiary structure of protein by altering Gibb’s free energy and entropy. Moreover, significant changes in SIFT, PHP-SNP, PolyPhen2, PROVEAN and RegulomeDB scores were also observed in mutations located in conserved domain of SOD1 protein. Multiple regression results were also suggesting the significant alterations in free energy and entropy for conserved domain mutations which were concordant with structural changes of SOD1 protein. Results of the study are suggesting the biological importance of location of mutation(s) which may derive the different disease phenotypes and must be dealt accordingly to provide precise therapy for ALS patients.

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