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Do genetic variants in the SPINK1 gene affect the level of serum PSTI?

Authors
  • Kume, Kiyoshi1
  • Masamune, Atsushi1
  • Ariga, Hiroyuki1
  • Hayashi, Shintaro1
  • Takikawa, Tetsuya1
  • Miura, Shin1
  • Suzuki, Noriaki1
  • Kikuta, Kazuhiro1
  • Hamada, Shin1
  • Hirota, Morihisa1
  • Kanno, Atsushi1
  • Shimosegawa, Tooru1
  • 1 Tohoku University Graduate School of Medicine, Division of Gastroenterology, 1-1 Seiryo-cho, Aoba-ku, Sendai, 980-8574, Japan , Sendai (Japan)
Type
Published Article
Journal
Journal of Gastroenterology
Publisher
Springer Japan
Publication Date
Apr 20, 2012
Volume
47
Issue
11
Pages
1267–1274
Identifiers
DOI: 10.1007/s00535-012-0590-3
Source
Springer Nature
Keywords
License
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Abstract

BackgroundThe serine protease inhibitor Kazal type 1 (SPINK1), also known as pancreatic secretory trypsin inhibitor (PSTI), is a peptide secreted by pancreatic acinar cells. Genetic studies have shown an association between SPINK1 gene variants and chronic pancreatitis or recurrent acute pancreatitis. The aim of this study was to clarify whether the SPINK1 variants affect the level of serum PSTI.MethodsOne hundred sixty-three patients with chronic pancreatitis or recurrent acute pancreatitis and 73 healthy controls were recruited. Serum PSTI concentrations were determined with a commercial radioimmunoassay kit.ResultsTen patients with the p.N34S variant, 7 with the IVS3+2T>C variant, two with both the p.N34S and the IVS3+2T>C variants, and one with the novel missense p.P45S variant in the SPINK1 gene were identified. The serum PSTI level in patients with no SPINK1 variants was 14.3 ± 9.6 ng/ml (mean ± SD), and that in healthy controls was 10.7 ± 2.2 ng/ml. The PSTI level in patients carrying the IVS3+2T>C variant (5.1 ± 3.4 ng/ml), but not in those with the p.N34S variant (8.9 ± 3.5 ng/ml), was significantly lower than that in the patients without the SPINK1 variants and the healthy controls. The serum PSTI level in the patient with the p.P45S variant was 4.9 ng/ml. Low levels of serum PSTI (<6.0 ng/ml) showed sensitivity of 80 %, specificity of 97 %, and accuracy of 96 % in the differentiation of IVS3+2T>C and p.P45S carriers from non-carriers.ConclusionSerum PSTI levels were decreased in patients with the IVS3+2T>C and p.P45S variants of the SPINK1 gene.

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