Affordable Access

DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand.

Authors
  • Keppler, M D
  • McKeen, C M
  • Zegrocka, O
  • Strekowski, L
  • Brown, T
  • Fox, K R
Type
Published Article
Journal
Biochimica et Biophysica Acta
Publisher
Elsevier
Publication Date
Oct 28, 1999
Volume
1447
Issue
2-3
Pages
137–145
Identifiers
PMID: 10542311
Source
Medline
License
Unknown

Abstract

We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold.

Report this publication

Statistics

Seen <100 times