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DNA-synthetic responses of fractionated human lymphocytes exposed to lymphocyte-derived mitogenic factor.

Authors
Type
Published Article
Journal
Scandinavian journal of immunology
Publication Date
Volume
5
Issue
10
Pages
1179–1184
Identifiers
PMID: 1087746
Source
Medline
License
Unknown

Abstract

Human peripheral lymphocytes that have been exposed to phytohemagglutinin (PHA) release mitogenic factors (MF) on subsequent incubation in fresh medium. In this investigation, MF-rich supernatants were analyzed for their ability to induce DNA synthesis in various fractions of peripheral human lymphocytes. MF was found to induce higher DNA synthesis in T-cell preparations that in unfractionated lymphoid preparations or B-cell-rich fractions. Evidence is presented showing that some types of non-T-cell can inhibit the MF response of T cells. These cells are nonadherent and nonphagocytic and require DNA synthesis to express their inhibitory activity. These results suggest that the regulation of the action of lymphocyte-derived MF is cell-mediated.

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