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DNA synergistic enzyme-mediated cascade reaction for homogeneous electrochemical bioassay.

Authors
  • Zhang, Yaxing1
  • Cao, Xiyue1
  • Deng, Ruixue1
  • Liu, Qingyun2
  • Xia, Jianfei3
  • Wang, Zonghua1
  • 1 College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Qingdaom, 266071, PR China. , (China)
  • 2 College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao, PR China. , (China)
  • 3 College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Qingdaom, 266071, PR China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Biosensors & bioelectronics
Publication Date
Jul 13, 2019
Volume
142
Pages
111510–111510
Identifiers
DOI: 10.1016/j.bios.2019.111510
PMID: 31319327
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Enzyme-mediated cascade reaction is applied to amplify signal and decrease the background because enzyme can catalyze inactive substrates into active substrates to generate the signal. In this work, Au nanoparticles, as signal probe, are used to load DNA probe and ALP for dual signal amplification. Based on enzyme-mediated cascade reaction, a homogeneous biosensor is constructed for bioassay by employing thrombin as target molecule. When the target is present in the solution, ALP catalyzes the PPi into Pi and then reacts with molybdate in conjunction with Pi in the DNA backbone to produce redox precipitates on the surface of the reduced graphene oxide modified electrode with the help of magnetic separation. Compared with the conventional heterogeneous biosensor, the immobilization-free strategy, proposed in this homogeneous biosensor, improves the sensitivity because of its lower steric hindrance. As a result, this biosensor displayed a great sensitivity with a wide linear range from 1 fM to 10 nM and a detection limit of 0.26 fM, providing a promise and easy operating method for various proteins detection. Copyright © 2019 Elsevier B.V. All rights reserved.

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