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DNA sequence and transcription of a DNA minicircle isolated from male-fertile sugar beet mitochondria.

Authors
  • Hansen, B M
  • Marcker, K A
Type
Published Article
Journal
Nucleic acids research
Publication Date
Jun 11, 1984
Volume
12
Issue
11
Pages
4747–4756
Identifiers
PMID: 6739294
Source
Medline
License
Unknown

Abstract

A minicircle consisting of 1440 bp was isolated from male fertile sugar beet mitochondria. The entire nucleotide sequence of the minicircle was determined. There are several stop codons in all three reading frames and it is therefore unlikely that the minicircle codes for a polypeptide product. However, the minicircle is transcribed yielding a major RNA product consisting of 850 bases in addition to two very minor RNA products of 1100 and 950 bases, respectively. All three RNA products are transcribed from the same strand. The starting point for transcription of the major RNA product was determined by S1 nuclease protection analysis.

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