Affordable Access

DNA preparation and efficient microsatellite analysis from insect hemolymph.

Authors
  • Gerken, T
  • Kurtz, J
  • Sauer, K P
  • Lubjuhn, T
Type
Published Article
Journal
Electrophoresis
Publication Date
Dec 01, 1998
Volume
19
Issue
18
Pages
3069–3070
Identifiers
PMID: 9932795
Source
Medline
License
Unknown

Abstract

A simple and time-saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1-5 microL of insect hemolymph in a Chelex 100-suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population structure and phylogenetic reconstruction can be conducted with minimal experimental influence.

Report this publication

Statistics

Seen <100 times