DNA preparation and efficient microsatellite analysis from insect hemolymph.
- Published Article
- Publication Date
Dec 01, 1998
A simple and time-saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1-5 microL of insect hemolymph in a Chelex 100-suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population structure and phylogenetic reconstruction can be conducted with minimal experimental influence.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/9932795