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DNA Polymerase of Chicken Embryo: Purification and Properties*

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PMC
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  • Biology
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Abstract

The almost complete purification of the previously reported DNA polymerase of chicken embryo is described; in the final isolation step the enrichment of the enzyme, which comprises about 0.01% of the protein extractable from 10-day-old embryos, is more than 9000-fold. The enzyme is a basic protein (pI = 9.15), of molecular weight about 27,000. It exhibits no nuclease activity, requires Mn2+ ion for activity, and may contain Zn. It is very sensitive to oxidation.

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