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DNA methylation analysis of the gene CDKN2B in Gallus gallus (chicken).

Authors
  • Gryzińska, Magdalena
  • Andraszek, Katarzyna
  • Jocek, Grzegorz
Type
Published Article
Journal
Folia biologica
Publication Date
Jan 01, 2013
Volume
61
Issue
3-4
Pages
165–171
Identifiers
PMID: 24279164
Source
Medline
License
Unknown

Abstract

Methylation is an epigenetic modification of DNA affecting gene expression without changing the structure of nucleotides. It plays a crucial role in the embryonic and post-embryonic development of living organisms. Methylation level is tissue and species-specific and changes with age. The study was aimed at identifying the methylation of the CDKN2B gene situated at locus bar in Polbar chickens on the 6th and 18th day of embryonic development using the MSP (methylation-specific PCR) method. Methylation was not detected in the promoter region of gene CDKN2B on the 6th and 18th day of embryonic development. As one of the five genes responsible for melanine activity in melanocytes and highly active, it can contribute to the production of this pigment. The present research broadens the current knowledge of the chicken epigenome and the mechanism of autosexing in birds.

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