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DNA Methylation Analysis of Cnr1 Gene Promoter.

Authors
  • D'Addario, Claudio1
  • Pucci, Mariangela2
  • 1 Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. , (Italy)
  • 2 Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. [email protected]. , (Italy)
Type
Published Article
Journal
Methods in molecular biology
Publisher
Clifton, N.J. : Humana Press
Publication Date
Jan 01, 2023
Volume
2576
Pages
373–384
Identifiers
DOI: 10.1007/978-1-0716-2728-0_31
PMID: 36152203
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

DNA methylation pattern could be considered a biomarker to be exploited for the study and management of several human diseases. In this chapter, detailed protocols are provided for two experimental approaches used for quantitative methylation analysis of bisulfite converted DNA: methylation-specific PCR (MSP) and pyrosequencing. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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