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DNA-dependent RNA-directed protein synthesis in vitro. IV. Peptide analysis of an in vitro and in vivo phi X174 structural protein.

Authors
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
Volume
67
Issue
1
Pages
13–17
Identifiers
PMID: 5272309
Source
Medline
License
Unknown

Abstract

An antibody-precipitable structural protein from the bacteriophage varphiX174 was purified from an in vitro protein-synthesizing system primed with the replicative DNA of the virus. This protein was trypsin digested in the presence of the differentially labeled analogous protein that was purified from the bacteriophage. The tryptic hydrolysate was subjected to high resolution peptide chromatography which revealed essentially identical patterns for the varphiX174 proteins synthesized in vitro and in vivo.

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