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DNA damage and repair in intact animals. Single-stranded regions in rat liver DNA following administration of dimethylnitrosamine.

Authors
  • Huang, P H
  • Stewart, B W
Type
Published Article
Journal
Cancer Letters
Publisher
Elsevier
Publication Date
May 01, 1976
Volume
1
Issue
5
Pages
263–268
Identifiers
PMID: 1016953
Source
Medline
License
Unknown

Abstract

Rat liver DNA was radioactively labelled by administration of [3H]thymidine following partial hepatectomy. Two weeks later, the rats were treated with the carcinogen, dimethylnitrosamine. DNA was isolated and fractionated by elution from benzoylated DEAE-cellulose with NaCl and caffeine solution. The caffeine-eluted fraction was increased by administration of dimethylnitrosamine. This increase was proportional to the dose of carcinogen injected and persisted for at least 24 h after administration of the carcinogen. These data, together with the results of hydroxyapatite chromatography, suggest that the DNA contains short single-stranded sections associated with much longer regions of native DNA.

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