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Diversity and phenotypic analyses of salt- and heat-tolerant wild bean Phaseolus filiformis rhizobia native of a sand beach in Baja California and description of Ensifer aridi sp. nov.

Authors
  • Rocha, Guadalupe1
  • Le Queré, Antoine2
  • Medina, Arturo1
  • Cuéllar, Alma1
  • Contreras, José-Luis3
  • Carreño, Ricardo1
  • Bustillos, Rocío1
  • Muñoz-Rojas, Jesús1
  • Villegas, María Del Carmen4
  • Chaintreuil, Clémence2
  • Dreyfus, Bernard2
  • Munive, José-Antonio5
  • 1 Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Av. San Claudio S/N, CP-72570, Puebla, Mexico. , (Mexico)
  • 2 IRD/CIRAD/UM2/Supagro-UR 040 Laboratoire des Symbioses Tropicales et Méditerranéennes, 34398, Montpellier, France. , (France)
  • 3 Facultad de Arquitectura, Benemérita Universidad Autónoma de Puebla, Av. San Claudio S/N, CP-72570, Puebla, Mexico. , (Mexico)
  • 4 Helyx Affaires SC, Rumania 923-2. Col. Portales-Sur. Alcaldía Benito Juárez, CP-03300, Cd. de México, Mexico. , (Mexico)
  • 5 Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Av. San Claudio S/N, CP-72570, Puebla, Mexico. [email protected] , (Mexico)
Type
Published Article
Journal
Archives of microbiology
Publication Date
Oct 28, 2019
Identifiers
DOI: 10.1007/s00203-019-01744-7
PMID: 31659382
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In northern Mexico, aridity, salinity and high temperatures limit areas that can be cultivated. To investigate the nature of nitrogen-fixing symbionts of Phaseolus filiformis, an adapted wild bean species native to this region, their phylogenies were inferred by MLSA. Most rhizobia recovered belong to the proposed new species Ensifer aridi. Phylogenetic analyses of nodC and nifH show that Mexican isolates carry symbiotic genes acquired through horizontal gene transfer that are divergent from those previously characterized among bean symbionts. These strains are salt tolerant, able to grow in alkaline conditions, high temperatures, and capable of utilizing a wide range of carbohydrates and organic acids as carbon sources for growth. This study improves the knowledge on diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico and further enlarges the spectrum of microsymbiont with which Phaseolus species can interact with, including cultivated bean varieties, notably under stressed environments. Here, the species Ensifer aridi sp. nov. is proposed as strain type of the Moroccan isolate LMR001T (= LMG 31426T; = HAMBI 3707T) recovered from desert sand dune.

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