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Diversification of DNA-Binding Specificity by Permissive and Specificity-Switching Mutations in the ParB/Noc Protein Family

Authors
  • Jalal, Adam S.B.1
  • Tran, Ngat T.1
  • Stevenson, Clare E.2
  • Chan, Elliot W.3
  • Lo, Rebecca1
  • Tan, Xiao1
  • Noy, Agnes3
  • Lawson, David M.2
  • Le, Tung B.K.1
  • 1 Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, UK
  • 2 Department of Biological Chemistry, John Innes Centre, Norwich NR4 7UH, UK
  • 3 Department of Physics, Biological Physical Sciences Institute, University of York, York YO10, UK
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Publication Date
Jul 21, 2020
Volume
32
Issue
3
Identifiers
DOI: 10.1016/j.celrep.2020.107928
PMID: 32698006
PMCID: PMC7383237
Source
PubMed Central
Keywords
License
Unknown

Abstract

Jalal et al. elucidate the molecular basis for how specific protein-DNA interactions can evolve, using ParB and Noc as models. Using X-ray crystallography and deep mutational scanning, they define protein-DNA interfaces and suggest that permissive mutations must be introduced before specificity-switching mutations to reprogram specificity.

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