EAE in rabbits was induced by means of inoculation of purified myelin of homologous spinal cord with complete Freund's adjuvant. The content of all the major lipid classes was studied by biochemical and histochemical methods in the different parts of spinal cord and in the brain stem in combination with morphological control for the demyelinating process presence. The most expressed myelin damage was found in the lumbar and sacral parts of spinal cord. In the same parts the content of phospholipids, cerebrosides, and free cholesterol decreased and cholesterol esters were shown to accumulate. Histochemical analysis supported these findings and revealed that the loss of lipids occurred directly in the demyelination foci. Changes in total ganglioside content and in ganglioside fractions ratio were not observed. In the brain stem neither morphological, nor biochemical changes were found. On the basis of these data it was concluded that pathological processes of periaxonal demyelination, induced by the sensitization with purified myelin, have not damaged neuronal structures and not involved the brain.