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Distribution of estrogen receptors in subfractions of hen oviduct chromatin.

Authors
  • Hemminki, K
Type
Published Article
Journal
Nucleic acids research
Publication Date
Jun 01, 1976
Volume
3
Issue
6
Pages
1499–1506
Identifiers
PMID: 183184
Source
Medline
License
Unknown

Abstract

Hen oviduct chromatin was digested with DNase II and separated into two fractions. The MgCl2 insoluble chromatin fraction (43% of the total DNA) was enriched in nucleosome-like particles, which sedimented at 11 S and contained 185 base pairs of DNA. The MgCl2 soluble chromatin fraction (5% of the total DNA) was characterized by 5 S and 14 S peaks in sucrose gradients; Estrogen receptors in the chromatin fractions were labelled with [3H] estradiol using the steroid exchange assay. The concentration of receptors in the MgCl2 soluble chromatin was 4;5 times higher than that in the MgCl2 insoluble chromatinmin sucrose gradient analysis the 11 S particles displayed a negligible specific radioactivity suggesting that estrogen receptors mainly bind to extranucleosomal chromatin.

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