Affordable Access

Distribution and consensus of branch point signals in eukaryotic genes: a computerized statistical analysis.

Authors
  • Harris, N L
  • Senapathy, P
Type
Published Article
Journal
Nucleic acids research
Publication Date
May 25, 1990
Volume
18
Issue
10
Pages
3015–3019
Identifiers
PMID: 2349097
Source
Medline
License
Unknown

Abstract

An intermediate stage in the process of eukaryotic RNA splicing is the formation of a lariat structure. It is anchored at an adenosine residue in intron between 10 and 50 nucleotides upstream of the 3' splice site. A short conserved sequence (the branch point sequence) functions as the recognition signal for the site of lariat formation. It has been generally assumed that the branch point is recognized mainly by the presence of its unique sequence where the lariat is formed. However, the known branch point consensus sequence is found to be distributed nearly randomly throughout the gene sequence with only a slightly higher frequency in the expected lariat region. Further, the known consensus sequence is found to be clearly inadequate to specify branch points. These observations have implications for understanding the mechanism of branch point recognition in the process of splicing, and the possible evolution of the branch point signal.

Report this publication

Statistics

Seen <100 times