Distribution of ClC-2 chloride channel in rat and human epithelial tissues.
- Authors
- Type
- Published Article
- Journal
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Publication Date
- Apr 26, 2002
- Volume
- 282
- Issue
- 4
- Identifiers
- DOI: 10.1152/ajpcell.00291.2001
- PMID: 11880269
- Source
- USPC - SET - SVS
- License
- Green
Abstract
The ubiquitous ClC-2 Cl(-) channel is thought to contribute to epithelial Cl(-) secretion, but the distribution of the ClC-2 protein in human epithelia has not been investigated. We have studied the distribution of ClC-2 in adult human and rat intestine and airways by immunoblotting and confocal microscopy. In the rat, ClC-2 was present in the lateral membranes of villus enterocytes and was predominant at the basolateral membranes of luminal colon enterocytes. The expression pattern of ClC-2 in the human intestine differed significantly, because ClC-2 was mainly detected in a supranuclear compartment of colon cells. We found significant expression of ClC-2 at the apex of ciliated cells in both rat and human airways. These results show that the distribution of ClC-2 in airways is consistent with participation of ClC-2 channels in Cl(-) secretion and indicate that extrapolation of results from studies of ClC-2 function in rat intestine to human intestine is not straightforward.
