Affordable Access

deepdyve-link
Publisher Website

Dissecting Cell Lineage Specification and Sex Fate Determination in Gonadal Somatic Cells Using Single-Cell Transcriptomics.

Authors
  • Stévant, Isabelle1
  • Kühne, Françoise2
  • Greenfield, Andy3
  • Chaboissier, Marie-Christine4
  • Dermitzakis, Emmanouil T1
  • Nef, Serge5
  • 1 Department of Genetic Medicine and Development, University of Geneva, 1211 Geneva, Switzerland; iGE3, Institute of Genetics and Genomics of Geneva, University of Geneva, 1211 Geneva, Switzerland; SIB, Swiss Institute of Bioinformatics, University of Geneva, 1211 Geneva, Switzerland. , (Switzerland)
  • 2 Department of Genetic Medicine and Development, University of Geneva, 1211 Geneva, Switzerland. , (Switzerland)
  • 3 Mammalian Genetics Unit, Medical Research Council, Harwell Institute, Oxfordshire OX11 0RD, UK. , (Mali)
  • 4 Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France. , (France)
  • 5 Department of Genetic Medicine and Development, University of Geneva, 1211 Geneva, Switzerland; iGE3, Institute of Genetics and Genomics of Geneva, University of Geneva, 1211 Geneva, Switzerland. Electronic address: [email protected] , (Switzerland)
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Publication Date
Mar 19, 2019
Volume
26
Issue
12
Identifiers
DOI: 10.1016/j.celrep.2019.02.069
PMID: 30893600
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Sex determination is a unique process that allows the study of multipotent progenitors and their acquisition of sex-specific fates during differentiation of the gonad into a testis or an ovary. Using time series single-cell RNA sequencing (scRNA-seq) on ovarian Nr5a1-GFP+ somatic cells during sex determination, we identified a single population of early progenitors giving rise to both pre-granulosa cells and potential steroidogenic precursor cells. By comparing time series single-cell RNA sequencing of XX and XY somatic cells, we provide evidence that gonadal supporting cells are specified from these early progenitors by a non-sex-specific transcriptomic program before pre-granulosa and Sertoli cells acquire their sex-specific identity. In XX and XY steroidogenic precursors, similar transcriptomic profiles underlie the acquisition of cell fate but with XX cells exhibiting a relative delay. Our data provide an important resource, at single-cell resolution, for further interrogation of the molecular and cellular basis of mammalian sex determination. Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.

Report this publication

Statistics

Seen <100 times