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Disruption of the Gene trx-m1 Impedes the Growth of Anabaena sp. PCC 7120 under Nitrogen Starvation.

Authors
  • Deschoenmaeker, Frï Dï Ric1
  • Mihara, Shoko1, 2
  • Niwa, Tatsuya3
  • Taguchi, Hideki3
  • Nomata, Jiro1, 2
  • Wakabayashi, Ken-Ichi1, 2
  • Hisabori, Toru1, 2
  • 1 Laboratory for Chemistry and Life Science, Institute for Innovative Research, Tokyo Institute of Technology, Nagatsuta-cho 4259-R1-8, Midori-ku, Yokohama, Japan. , (Japan)
  • 2 Department of Life Science, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, Yokohama, Japan. , (Japan)
  • 3 Cell Biology Center, Tokyo Institute of Technology, Nagatsuta-cho, 4259-S2-19 Midori-ku Yokohama, Japan. , (Japan)
Type
Published Article
Journal
Plant and Cell Physiology
Publisher
Oxford University Press
Publication Date
Jul 01, 2019
Volume
60
Issue
7
Pages
1504–1513
Identifiers
DOI: 10.1093/pcp/pcz056
PMID: 31038682
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Cyanobacteria possess a sophisticated photosynthesis-based metabolism with admirable plasticity. This plasticity is possible via the deep regulation network, the thiol-redox regulations operated by thioredoxin (hereafter, Trx). In this context, we characterized the Trx-m1-deficient mutant strain of Anabaena sp., PCC 7120 (shortly named A.7120), cultivated under nitrogen limitation. Trx-m1 appears to coordinate the nitrogen response and its absence induces large changes in the proteome. Our data clearly indicate that Trx-m1 is crucial for the diazotrophic growth of A.7120. The lack of Trx-m1 resulted in a large differentiation of heterocysts (>20% of total cells), which were barely functional probably due to a weak expression of nitrogenase. In addition, heterocysts of the mutant strain did not display the usual cellular structure of nitrogen-fixative cells. This unveiled why the mutant strain was not able to grow under nitrogen starvation. � The Author(s) 2019. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: [email protected]

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