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Disposable Nafion-Coated Single-Walled Carbon Nanotube Test Strip for Electrochemical Quantitative Determination of Acetaminophen in a Finger-Prick Whole Blood Sample

Authors
  • Wester, Niklas1
  • Mikladal, Bjørn F.2
  • Varjos, Ilkka2
  • Peltonen, Antti1
  • Kalso, Eija3, 4
  • Lilius, Tuomas3, 4
  • Laurila, Tomi1
  • Koskinen, Jari1
  • 1 Aalto University, Finland , (Finland)
  • 2 Canatu Oy, Finland , (Finland)
  • 3 University of Helsinki, Finland , (Finland)
  • 4 University of Helsinki and Helsinki University Hospital, Finland , (Finland)
Type
Published Article
Journal
Analytical Chemistry
Publisher
American Chemical Society
Publication Date
Aug 26, 2020
Volume
92
Issue
19
Pages
13017–13024
Identifiers
DOI: 10.1021/acs.analchem.0c01857
PMID: 32842738
PMCID: PMC7547857
Source
PubMed Central
License
Unknown

Abstract

A disposable electrochemical test strip for the quantitative point-of-care (POC) determination of acetaminophen (paracetamol) in plasma and finger-prick whole blood was fabricated. The industrially scalable dry transfer process of single-walled carbon nanotubes (SWCNTs) and screen printing of silver were combined to produce integrated electrochemical test strips. Nafion coating stabilized the potential of the Ag reference electrode and enabled the selective detection in spiked plasma as well as in whole blood samples. The test strips were able to detect acetaminophen in small 40 μL samples with a detection limit of 0.8 μM and a wide linear range from 1 μM to 2 mM, well within the required clinical range. After a simple 1:1 dilution of plasma and whole blood, a quantitative detection with good recoveries of 79% in plasma and 74% in whole blood was achieved. These results strongly indicate that these electrodes can be used directly to determine the unbound acetaminophen fraction without the need for any additional steps. The developed test strip shows promise as a rapid and simple POC quantitative acetaminophen assay.

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