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Discovery of a Novel Multi-Strains Probiotic Formulation with Improved Efficacy toward Intestinal Inflammation

Authors
  • Biagioli, Michele1
  • Carino, Adriana1
  • Di Giorgio, Cristina1
  • Marchianò, Silvia1
  • Bordoni, Martina1
  • Roselli, Rosalinda
  • Distrutti, Eleonora
  • Fiorucci, Stefano1
  • 1 (M.B.)
Type
Published Article
Journal
Nutrients
Publisher
MDPI AG
Publication Date
Jun 30, 2020
Volume
12
Issue
7
Identifiers
DOI: 10.3390/nu12071945
PMID: 32629887
PMCID: PMC7400193
Source
PubMed Central
Keywords
License
Green

Abstract

Dysbiosis is commonly detected in patients with inflammatory bowel disease (IBD), supporting the concept that a dysregulated immune reaction to bacterial antigens has a pathogenic role in the development of intestinal inflammation. In the present study, we have investigated the beneficial effects of a novel probiotic formulation assembled by combining four probiotics ( Streptococcus thermophilus , Lactobacillus casei , Bifidobacterium breve, Bifidobacterium animalis subsp. Lactis ) with Bacillus subtilis , a Gram-positive bacterium, with extensive bio-applications. Mice rendered colitic by administration of TNBS or DSS were administered with Bacillus subtilis alone, Vivomixx® or the novel Five strains formulation. Vivomixx® attenuated the severity of inflammation and reduced the development of signs and symptoms of colitis in both models. Adding Bacillus subtilis to Vivomixx® improved the beneficial effects of the bacterial therapy. The novel Five strains formulation was as effective as Vivomixx® in reducing the development of signs and symptoms of colitis and reduced the expression of pro-inflammatory mediators including Il-6 and Tnf-α while increased the expression of Il-10 mRNA and the number of Treg. In summary, we have shown that a novel Five strains probiotics formulation exerts beneficial effects on two chemical models of colitis, establishing Bacillus subtilis as a probiotic in rodent models of inflammation.

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