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Directed termination PCR: a one-step approach to mutation detection.

Authors
  • J Chen
  • P D Hebert
Publication Date
Mar 15, 1998
Source
PMC
Keywords
License
Unknown

Abstract

We describe a novel PCR-based method that allows the generation of nested termination fragments by integrating both selective DNA amplification and directed chain termination into a single PCR reaction. These termination fragments can be examined for sequence variation in either denaturing or non-denaturing polyacrylamide gels. This method provides a one-step and highly effective approach for the detection of both insertions/deletions and single base pair substitutions in sequences up to 1 kb in length.

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