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Direct electric current treatment modifies mitochondrial function and lipid body content in the A549 cancer cell line.

Authors
  • Holandino, Carla1
  • Teixeira, Cesar Augusto Antunes2
  • de Oliveira, Felipe Alves Gomes2
  • Barbosa, Gleyce Moreno2
  • Siqueira, Camila Monteiro3
  • Messeder, Douglas Jardim4
  • de Aguiar, Fernanda Silva2
  • da Veiga, Venicio Feo5
  • Girard-Dias, Wendell6
  • Miranda, Kildare6
  • Galina, Antonio4
  • Capella, Marcia Alves Marques7
  • Morales, Marcelo Marcos8
  • 1 Laboratory of Multidisciplinary Pharmaceutical Sciences, College of Pharmacy, Center of Health Sciences (CCS), Federal University do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: [email protected] , (Brazil)
  • 2 Laboratory of Multidisciplinary Pharmaceutical Sciences, College of Pharmacy, Center of Health Sciences (CCS), Federal University do Rio de Janeiro, Rio de Janeiro, Brazil. , (Brazil)
  • 3 Federal Institute of Education, Science and Technology of Rio de Janeiro, Rio de Janeiro, Brazil. , (Brazil)
  • 4 Laboratory of Bioenergetics and Mitochondrial Physiology, Institute of Medical Biochemistry, CCS, Federal University do Rio de Janeiro, Brazil. , (Brazil)
  • 5 Laboratory of Electron Microscopy, Institute of Microbiology Prof. Paulo de Góes (IMPPG), CCS, UFRJ, Rio de Janeiro, Brazil. , (Brazil)
  • 6 Laboratory of Cellular Ultrastructure Hertha Meyer, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. , (Brazil)
  • 7 Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. , (Brazil)
  • 8 Laboratory of Molecular and Cellular Physiology, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. , (Brazil)
Type
Published Article
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
Publication Date
Oct 01, 2016
Volume
111
Pages
83–92
Identifiers
DOI: 10.1016/j.bioelechem.2016.05.004
PMID: 27243447
Source
Medline
Keywords
License
Unknown

Abstract

Electrochemical therapy (EChT) entails treatment of solid tumors with direct electric current (DC). This work evaluated the specific effects of anodic flow generated by DC on biochemical and metabolic features of the A549 human lung cancer cell line. Apoptosis was evaluated on the basis of caspase-3 activity and mitochondrial transmembrane potential dissipation. Cell morphology was analyzed using transmission electron microscopy, and lipid droplets were studied through morphometric analysis and X-ray qualitative elemental microanalysis. High-resolution respirometry was used to assess mitochondrial respiratory parameters. Results indicated A549 viability decreased in a dose-dependent manner with a prominent drop between 18 and 24h after treatment (p<0.001), together with a two-fold increase in caspase-3 activity. AF-treatment induced a significantly increase (p<0.01) in the cell number with disrupted mitochondrial transmembrane potential. Furthermore, treated cells demonstrated important ultrastructural mitochondria damage and a three-fold increase in the cytoplasmic lipid bodies' number, quantified by morphometrical analyses. Conversely, 24h after treatment, the cells presented a two-fold increase of residual oxygen consumption, accounting for 45.3% of basal oxygen consumption. These results show remarkable alterations promoted by anodic flow on human lung cancer cells which are possibly involved with the antitumoral effects of EChT.

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