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Differentiation of peripheral blood monocytes into dendritic cells.

Authors
Type
Published Article
Journal
Current protocols in immunology / edited by John E. Coligan ... [et al.]
1934-3671
Publication Date
Volume
Chapter 22
Identifiers
DOI: 10.1002/0471142735.im22f04s67
PMID: 18432951
Source
Medline
License
Unknown

Abstract

Dendritic cells (DCs) are potent antigen-presenting cells (APC) that are important in the initiation and control of cellular immune responses. Commonly used in T cell-stimulation experiments, DCs are typically "matured" in vitro with microbial products or proinflammatory cytokines, and then loaded with antigens from any number of sources, including peptides, whole proteins, cell lysates, RNA, microbes, or killed tumor cells. This unit presents a simple and commonly used method for the generation of mature human dendritic cells--differentiating them from peripheral blood monocytes.

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