Affordable Access

deepdyve-link deepdyve-link
Publisher Website

Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS.

Authors
  • Sanders, Melinda E
  • Dias, Eduardo C
  • Xu, Baogang J
  • Mobley, James A
  • Billheimer, Dean
  • Roder, Heinrich
  • Grigorieva, Julia
  • Dowsett, Mitchell
  • Arteaga, Carlos L
  • Caprioli, Richard M
Type
Published Article
Journal
Journal of proteome research
Publication Date
Apr 01, 2008
Volume
7
Issue
4
Pages
1500–1507
Identifiers
DOI: 10.1021/pr7008109
PMID: 18386930
Source
Medline
License
Unknown

Abstract

We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the m/ z features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.

Report this publication

Statistics

Seen <100 times