Affordable Access

deepdyve-link
Publisher Website

Differentially expressed proteins in human MCF-7 breast cancer cells sensitive and resistant to paclitaxel.

Authors
  • Pavlíková, Nela1
  • Bartoňová, Irena2
  • Balušíková, Kamila2
  • Kopperova, Dana3
  • Halada, Petr4
  • Kovář, Jan2
  • 1 Department of Cell & Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic. Electronic address: [email protected] , (Czechia)
  • 2 Department of Cell & Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic. , (Czechia)
  • 3 Department of Cell & Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czech Republic. Electronic address: [email protected] , (Czechia)
  • 4 Laboratory of Molecular Structure Characterization, Institute of Microbiology,v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic. , (Czechia)
Type
Published Article
Journal
Experimental Cell Research
Publisher
Elsevier
Publication Date
Apr 10, 2015
Volume
333
Issue
1
Pages
1–10
Identifiers
DOI: 10.1016/j.yexcr.2014.12.005
PMID: 25557873
Source
Medline
Keywords
License
Unknown

Abstract

Resistance of cancer cells to chemotherapeutic agents is one of the main causes of treatment failure. In order to detect proteins potentially involved in the mechanism of resistance to taxanes, we assessed differences in protein expression in MCF-7 breast cancer cells that are sensitive to paclitaxel and in the same cells with acquired resistance to paclitaxel (established in our lab). Proteins were separated using two-dimensional electrophoresis. Changes in their expression were determined and proteins with altered expression were identified using mass spectrometry. Changes in their expression were confirmed using western blot analysis. With these techniques, we found three proteins expressed differently in resistant MCF-7 cells, i.e., thyroid hormone-interacting protein 6 (TRIP6; upregulated to 650%), heat shock protein 27 (HSP27; downregulated to 50%) and cathepsin D (downregulated to 28%). Silencing of TRIP6 expression by specific siRNA leads to decreased number of grown resistant MCF-7 cells. In the present study we have pointed at some new directions in the studies of the mechanism of resistance to paclitaxel in breast cancer cells.

Report this publication

Statistics

Seen <100 times