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On the Differential Roles of Mg2+, Zn2+, and Cu2+ in the Equilibrium of β-N-Methyl-Amino-L-Alanine (BMAA) and its Carbamates.

Authors
  • Diaz-Parga, Pedro1
  • Goto, Joy J2
  • Krishnan, V V3, 4
  • 1 Department of Chemistry, California State University, Fresno, CA, 93740, USA.
  • 2 Department of Chemistry, California State University, Fresno, CA, 93740, USA. [email protected]
  • 3 Department of Chemistry, California State University, Fresno, CA, 93740, USA. [email protected]
  • 4 Department of Pathology & Laboratory Medicine, University of California Davis, Davis, CA, 95616, USA. [email protected]
Type
Published Article
Journal
Neurotoxicity Research
Publisher
Springer-Verlag
Publication Date
Feb 01, 2021
Volume
39
Issue
1
Pages
6–16
Identifiers
DOI: 10.1007/s12640-019-00157-0
PMID: 31955368
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

β-N-methyl-amino-L-alanine (BMAA) in the presence of bicarbonate (HCO3-) undergoes structural modifications generating two carbamate species, α-carbamate and β-carbamate forms of BMAA. The chemical structure of BMAA and BMAA-carbamate adducts strongly suggest they may interact with divalent metal ions. The ability of BMAA to cross the blood-brain barrier and possibly interact with divalent metal ions may augment the neurotoxicity of these molecules. To understand the effects of divalent metal ions (Mg2+, Zn2+, and Cu2+) on the overall dynamic equilibrium between BMAA and its carbamate adducts, a systematic study using nuclear magnetic resonance (NMR) is presented. The chemical equilibria between BMAA, its carbamate adducts, and each of the divalent ions were studied using two-dimensional chemical exchange spectroscopy (EXSY). The NMR results demonstrate that BMAA preferentially interacts with Zn2+ and Cu2+, causing an overall reduction in the production of carbamate species by altering the dynamic equilibria. The NMR-based spectral changes due to the BMAA interaction with Cu2+ is more drastic than with the Zn2+, under the same stoichiometric ratios of BMAA and the individual divalent ions. However, the presence of Mg2+ does not significantly alter the dynamic equilibria between BMAA and its carbamate adducts. The NMR-based results are further validated using circular dichroism (CD) spectroscopy, observing the n ➔ π interaction in the complex formation of BMAA and the divalent metal ions, with additional verification of the interaction with Cu2+ using UV-Vis spectroscopy. Our results demonstrate that BMAA differentially interacts with divalent metal ions (Mg2+ < Zn2+ < Cu2+), and thus alters the rate of formation of carbamate products. The equilibria between BMAA, the bicarbonate ions, and the divalent metal ions may alter the total population of a specific form of BMAA-ion complex at physiological conditions and, therefore, add a level of complexity of the mechanisms by which BMAA acts as a neurotoxin.

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