Affordable Access

Publisher Website

Differential response of adipose tissue gene and protein expressions to 4- and 8-week administration of β-guanidinopropionic acid in mice.

  • Kato, Hisashi1
  • Masuda, Shinya1
  • Ohira, Tomotaka2
  • Ohira, Luna3
  • Takakura, Hisashi1, 4
  • Ohira, Yoshinobu1, 4, 5, 6
  • Izawa, Tetsuya1, 4, 5
  • 1 Faculty and Graduate School of Health and Sports Science, Doshisha University, Kyotanabe, Japan. , (Japan)
  • 2 Chiben Gakuen Nara College, Kashiba, Japan. , (Japan)
  • 3 Faculty of Health and Well-being, Kansai University, Sakai, Japan. , (Japan)
  • 4 Research Center for Adipocyte and Muscle Science, Doshisha University, Kyotanabe, Japan. , (Japan)
  • 5 Research Center for Space Medical Science, Doshisha University, Kyotanabe, Japan. , (Japan)
  • 6 Graduate School of Medicine, Osaka University, Toyonaka, Japan. , (Japan)
Published Article
Physiological Reports
Wiley (Physiological Reports)
Publication Date
Mar 01, 2018
DOI: 10.14814/phy2.13616
PMID: 29512301


β-Guanidinopropionic acid (β-GPA) feeding inhibits growth-associated gain of body mass. It remains unknown, however, whether and how β-GPA feeding affects growth-associated increase in white adipose tissue (WAT) mass. We examined the effects of 4- and 8-week β-GPA feeding on serum myostatin levels and expression of genes and proteins related to adipogenesis, lipolysis, and liposynthesis in epididymal WAT (eWAT) and brown adipose tissue (BAT) in 3-week-old, juvenile male mice. Body, eWAT, and muscle weights were significantly lower in β-GPA-fed mice than in controls after feeding. Four- but not 8-week-β-GPA feeding increased the serum myostatin level. Incubation of C2C12 myotubes with β-GPA (1 mM) significantly promoted myostatin mRNA expression. The protein expression of peroxisome proliferator-activated receptor gamma coactivator 1 α (PGC-1α) and peroxisome proliferator-activated receptor α (PPARα) was up-regulated in GPAF eWAT at week 4, but down-regulated at week 8. There was no significant difference in the protein expression of adipocyte triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) between groups in eWAT. In BAT, no significant difference was found in the protein expression of PGC-1α, PPARα, ATGL, and HSL between β-GPA-fed and control mice, whereas that of FAS and ACC was significantly lower in β-GPA-fed mice at week 8. Uncoupling protein 1 was expressed higher in β-GPA-fed mice both at weeks 4 and 8 than that in controls. Thus, the mechanism by which β-GPA feeding in early juvenile mice inhibits growth-associated increase in eWAT mass may differ between early and later periods of growth. © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

Report this publication


Seen <100 times