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Differential involvement of phospholipase C isozymes in Alzheimer's disease.

Authors
  • Shimohama, S
  • Matsushima, H
  • Fujimoto, S
  • Takenawa, T
  • Taniguchi, T
  • Kameyama, M
  • Kimura, J
Type
Published Article
Journal
Gerontology
Publication Date
Jan 01, 1995
Volume
41 Suppl 1
Pages
13–19
Identifiers
PMID: 8537014
Source
Medline
License
Unknown

Abstract

Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in signal transduction. We demonstrated that an antibody to an isozyme of PLC, PLC-delta 1, produced intense staining of neurofibrillary tangles (NFT) in the brains of patients with Alzheimer's disease (AD). Western blot analysis using a specific antibody for PLC-delta 1 showed that the concentration of PLC-delta 1 protein was significantly higher in the cytosolic fraction of AD cortical tissues than in controls. The activity of PLC-delta 1, which hydrolyzes phosphatidylinositol, was not significantly different in the Alzheimer and control cytosolic fraction, indicating that the specific activity of PLC-delta 1 is decreased in Alzheimer brains. Western blot analysis also showed that the concentration of PLC-delta 1 and PLC-gamma 1 was significantly lower in the membraneous fraction of AD cortical tissues than in controls. These results suggest a differential involvement of PLC isozymes in AD.

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