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Differential expression of starch and sucrose metabolic genes linked to varying biomass yield in Miscanthus hybrids

Authors
  • De Vega, Jose J.1
  • Peel, Ned1
  • Purdy, Sarah J.2, 3
  • Hawkins, Sarah2
  • Donnison, Lain2
  • Dyer, Sarah1, 4
  • Farrar, Kerrie2
  • 1 Earlham Institute, Norwich, NR4 7UZ, UK , Norwich (United Kingdom)
  • 2 Aberystwyth University, Aberystwyth, SY23 3EE, UK , Aberystwyth (United Kingdom)
  • 3 Chief Scientist’s Branch, Locked Bag 21, Orange, NSW, 2800, Australia , Orange (Australia)
  • 4 NIAB, Cambridge, CB3 0LE, UK , Cambridge (United Kingdom)
Type
Published Article
Journal
Biotechnology for Biofuels
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Apr 19, 2021
Volume
14
Issue
1
Identifiers
DOI: 10.1186/s13068-021-01948-4
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundMiscanthus is a commercial lignocellulosic biomass crop owing to its high biomass productivity and low chemical input requirements. Within an interspecific Miscanthus cross, progeny with high biomass yield were shown to have low concentrations of starch and sucrose but high concentrations of fructose. We performed a transcriptional RNA-seq analysis between selected Miscanthus hybrids with contrasting values for these phenotypes to clarify how these phenotypes are genetically controlled.ResultsWe observed that genes directly involved in the synthesis and degradation of starch and sucrose were down-regulated in high-yielding Miscanthus hybrids. At the same time, glycolysis and export of triose phosphates were up-regulated in high-yielding Miscanthus hybrids. These differentially expressed genes and biological functions were regulated by a well-connected network of less than 25 co-regulated transcription factors.ConclusionsOur results evidence a direct relationship between high expression of essential enzymatic genes in the starch and sucrose pathways and co-expression with their transcriptional regulators, with high starch concentrations and lower biomass production. The strong interconnectivity between gene expression and regulators, chemotype and agronomic traits opens the door to use the expression of well-characterised genes associated with carbohydrate metabolism, particularly in the starch and sucrose pathway, for the early selection of high biomass-yielding genotypes from large Miscanthus populations.

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