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Differential effects on T cell and NK cell development by tissue-specific expression of H-2D(d) transgene.

Authors
  • Johansson, M H
  • Bieberich, C
  • Kâse-Sjöström, A
  • Yoshioka, T
  • Höglund, E
  • Christy, B A
  • Scangos, G
  • Kärre, K
  • Jay, G
  • Höglund, P
Type
Published Article
Journal
European journal of immunology
Publication Date
Feb 01, 2000
Volume
30
Issue
2
Pages
525–533
Identifiers
PMID: 10671208
Source
Medline
License
Unknown

Abstract

The effect of tissue-specific expression of the MHC class I molecule H-2D(d) on T cell and NK cell specificity was studied in transgenic mice expressing the H-2D(d) gene under the control of the mouse metallothionein-I promoter. MTD mice expressed high amounts of H-2D(d) in the liver, intestine and testis, but only minute amounts in the thymus, spleen and kidney. Zinc administration resulted in a 1.5- and 8.5-fold increase in H-2D(d) expression in the liver and the intestine, respectively, but did not affect expression in the other organs tested. T cell tolerance developed towards H-2D(d) in MTD mice, even in the absence of zinc. In contrast, NK cell-mediated natural resistance against lymphoma grafts was not seen in MTD mice, despite zinc administration. NK cells in MTD mice also failed to develop self tolerance to H-2D(d). The lack of functional effects did not result from inability of NK cells in MTD mice to interact with H-2D(d), as down-regulation of Ly49A receptor expression was observed on liver NK cells in MTD mice. Our data reveal a difference between T cells and NK cells in their requirements for MHC class I molecules in specificity development.

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