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Differential Effects of 5-Methylmercapto-2′-Deoxyuridine on the Replication of Herpes Simplex Virus Type 1 in Two Cell Systems

Authors
  • Robert Hardi
  • R. G. Hughes
  • Y. K. Ho
  • K. C. Chadha
  • T. J. Bardos
Publication Date
Oct 01, 1976
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

5-Methylmercapto-2′-deoxyuridine (MeMUdR), a structural analogue of thymidine (TdR), inhibits herpes simplex virus type 1 production in mouse L (Lb) cells at concentrations that are not inhibitory to viral growth in monkey kidney (CV-1) cells. It is moderately toxic to Lb cells but not to CV-1 cells at a concentration that causes 95% inhibition of viral replication in Lb cells. MeMUdR is incorporated into cellular and viral deoxyribonucleic acid (DNA) in both systems, but to a significantly higher level (compared with thymidine) in Lb cells. These results indicate that MeMUdR is a substrate for enzymes leading to DNA synthesis and suggest that the biological function of herpes simplex virus type 1 DNA is impaired only when the incorporation of MeMUdR into the DNA reaches a relatively high level.

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