Affordable Access

Differential effects of cladribine and gemcitabine on erythroid and granulocytic progenitors from patients with chronic myeloid leukemia.

Authors
  • Schirmer, M
  • Geisen, F
  • Tiefenthaler, M
  • Konwalinka, G
Type
Published Article
Journal
Leukemia Research
Publisher
Elsevier
Publication Date
Dec 01, 1999
Volume
23
Issue
12
Pages
1121–1126
Identifiers
PMID: 10613357
Source
Medline
License
Unknown

Abstract

Chronic myeloid leukemia (CML) is a clonal neoplastic disease that originates in a pluripotent stem cell. Selection of normal progenitors by graft-purging may improve the outcome after autologous transplantation. In our methylcellulose assays, the nucleoside analogs cladribine (2-CdA) and gemcitabine (dFdC) showed more prominent inhibitory effects on CML than normal bone marrow (BM) progenitors. For dFdC, however, long-term incubations were necessary to achieve complete inhibition. Deoxycytidine kinase, the key enzyme of both 2-CdA and dFdC metabolisms, was only partially responsible for this differential sensitivity. We suggest that 2-CdA and dFdC might be helpful in purging of CML BM cells before autologous BM transplantation. Further studies on more primitive cells are warranted.

Report this publication

Statistics

Seen <100 times