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Differential binding properties of B7-H1 and B7-DC to programmed death-1.

Authors
  • Youngnak, Pornpan
  • Kozono, Yuko
  • Kozono, Haruo
  • Iwai, Hideyuki
  • Otsuki, Noriko
  • Jin, Hisayo
  • Omura, Ken
  • Yagita, Hideo
  • Pardoll, Drew M
  • Chen, Lieping
  • Azuma, Miyuki
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Aug 01, 2003
Volume
307
Issue
3
Pages
672–677
Identifiers
PMID: 12893276
Source
Medline
License
Unknown

Abstract

Programmed death-1 (PD-1) is a negative regulatory receptor expressed on activated T and B cells. Two ligands for PD-1, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified, but their binding properties have not been characterized yet. In this study, we generated soluble Ig fusion proteins of these molecules and examined the kinetics and relative affinities of the interactions between B7-H1 or B7-DC and PD-1 by flow cytometry and surface plasmon resonance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity and had different association/dissociation kinetics compared with the interaction of B7-H1/PD-1. Our results suggest that the differential binding properties of B7-H1 and B7-DC may be responsible for differential contributions of these two PD-1 ligands to immune responses.

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