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Differential accessibility of bilirubin to erythrocyte membrane vesicles bearing different structural features.

Authors
  • Rashid, H
  • Ali, M K
  • Tayyab, S
Type
Published Article
Journal
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Publisher
Elsevier BV
Publication Date
Dec 01, 2000
Volume
127
Issue
3
Pages
345–350
Identifiers
PMID: 11246506
Source
Medline
License
Unknown

Abstract

Interaction of bilirubin with different types of erythrocyte membrane vesicles such as unsealed, heterogeneous, sealed and inside-out membrane vesicles prepared from human and goat erythrocytes was studied. Out of various types of membrane vesicles, in both species, unsealed membrane vesicles bound quantitatively higher amounts of bilirubin followed by heterogeneous and sealed membrane vesicles whereas inside-out membrane vesicles bound the lowest amount of bilirubin. These differences in the amount of bound bilirubin to different membrane vesicles were correlated well with the percentage accessibility of sialic acid to neuraminidase in these membranes suggesting that bilirubin bound preferentially to the outer layer of erythrocyte membranes than the inner layer. Further, membrane vesicles prepared from human erythrocytes bound higher amounts of bilirubin than those prepared from goat erythrocytes. This can be ascribed to different phospholipid composition of these membranes.

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