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Different electrically charged proteins result in diverse bacterial transport behaviors in porous media.

Authors
  • Wu, Dan1
  • He, Lei1
  • Ge, Zhi1
  • Tong, Meiping2
  • Kim, Hyunjung3
  • 1 The Key Laboratory of Water and Sediment Sciences, Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, PR China. , (China)
  • 2 The Key Laboratory of Water and Sediment Sciences, Ministry of Education, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, PR China. Electronic address: [email protected] , (China)
  • 3 Department of Mineral Resources and Energy Engineering, Chonbuk National University, Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea. , (North Korea)
Type
Published Article
Journal
Water research
Publication Date
Oct 15, 2018
Volume
143
Pages
425–435
Identifiers
DOI: 10.1016/j.watres.2018.06.070
PMID: 29986251
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The influence of proteins on bacterial transport and deposition behaviors in quartz sand was examined in both NaCl (10 and 25 mM) and CaCl2 solutions (1.2 and 5 mM). Bovine Serum Albumin (BSA) and bovine trypsin were used to represent negatively and positively charged proteins in natural aquatic systems, respectively. The presence of negatively charged BSA in suspensions increased the transport and decreased bacterial deposition in quartz sand, regardless of the ionic strength and ion types. Whereas, positively charged trypsin inhibited the transport and enhanced bacterial deposition under all experimental conditions. The potential mechanisms controlling the changes of bacterial transport behaviors varied for different charged proteins. The steric repulsion resulting from BSA adsorption onto both bacteria and quartz sand was found to play a dominant role in the transport and deposition of bacteria in porous media with BSA copresent in suspension. BSA adsorption onto bacterial surfaces and competition for deposition sites onto sand surfaces (adsorption of quartz sand surfaces) contributed to the increased cell transport with BSA in suspension. In contrast, the attractive patch-charged interaction induced by the adsorption of trypsin onto both bacteria and quartz sand had great contribution to the decreased bacterial transport in porous media with trypsin copresent in suspension. The increase in bacteria size, and the adsorption of trypsin onto cell surfaces (resulting in less negative cell surface charge) and quartz sand surfaces (providing extra deposition sites) were found to be the main contributors to the decreased transport and increased deposition of bacteria in quartz sand with trypsin in suspension. Copyright © 2018 Elsevier Ltd. All rights reserved.

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